Development of Immunofluorescence Reader Based on Microfluidic Chip

This paper introduces the technical principle of microfluidic immunofluorescence interpreters, and then introduces the development method of reader from the aspects of the system, optics, hardware and software algorithms. Firstly, the photoelectric sensor of the reader is designed, and then the software algorithms such as filtering the data scanned by the sensor and finding the curve peak are researched. Finally, the quantitative concentration result of the sample liquid is obtained. In this paper, a quantitative experimental test on clinical PSA standard samples was performed, and the results indicate the correlation coefficient between the reader and the FREND reader of NanoEnTek was more than 99%, and the CV is less than 10%.