Evaluation of Material Permeability of Type I Collagen Hydrogel

Objective To establish an experimental method for evaluating material permeability of type I collagen hydrogels. Methods Using BSA-FITC as an indicator, by combining BSA-FITC with PBS they were used as permeability media, and using transwell load hydrogen sample to detect BSA-FITC transparent rate. Results In the concentration range of 100 μg·mL^-1~0.781 μg·mL^-1, the standard curve R² ≥ 0.99, Lower Limit of Quantity (LLOQ) is 3.125 μg·mL^-1, RSD <5%, detection recovery rate is in the range of 80%~120%. Conclusion In this study, we established an experimental method for evaluating material permeability of hydrogel. The BSA-FITC transparent rate of type I collagen hydrogel was 100% at 28 h.