Abstract: Naked-eye 3D display technology has excellent 3D visual effects and does not require wearable devices assistance. It can present the depth, position and complex structure information of 3D medical images, allowing viewers to obtain information about tissues and organs from different points, reducing cognitive load, contributing to medical teaching and opening up innovative methods for planning and diagnosis. Naked-eye 3D augmented reality display can display medical images in real 3D space, achieving virtual and real vision. It helps a lot to medical research. The applications of naked-eye 3D display technology in three major aspects of medical diagnosis, clinical surgery and rehabilitation training is reviewed in the study. It provides the direction for the subsequent research in medical field, thus assisting medical research and improving medical practice.