Abstract: This study aims to discuss the factors affecting the hemolytic properties of α-cyanoacrylate glues for surgical use. The results showed that the different extractions, test methods, pH value, rapid solidification and the extract ratio were the main factors affecting the hemolytic properties. The choose of PBS as the extraction for haemolysis test maybe was more suitable than physiological saline. It was recommended that using both direct contact methods and indirect contact methods should be more comprehensive for hemolytic evaluation.