Abstract: The emergence of smart phones has not only brought extensive changes to people’s way of life, but also provided a new research platform for the expansion and application of science and technology. Technicians have combined immunoassay methods with smart phone sensing technology to develop a variety of smartphone-based biological sample analysis and detection sensing systems, promoting the application of immunoassay methods in POCT fields. In this review, we summarize the researches and applications of smart-phone in the field of immune analysis. According to the different sensors and detection objects, these applications can be divided into four aspects: camera-based spectrometer, camera-based enzyme reader, camera-based strip reader and spectrophotometer based on environmental light sensor. This study also briefly discusses some limitations of current smart phone application in immune analysis, and prospects the future applicability of smart phone sensing system.