Abstract: Objective The Python research environment for radiation therapy (PyRERT) is a set of business software for hospital physicists to conduct radiation therapy research. Methods Choose the open source Enthought Tool Suite（ETS） as the core external dependency library of PyRERT. PyRERT is divided into base layer, content layer and interaction layer, and each layer is composed of different functional modules. Results PyRERT V1.0 provide a good development environment for scientific research programming in DICOM RT file processing, batch processing of water tank scan data, digital phantom creation, 3D medical image volume visualization, virtual radiotherapy equipment driver, and film scan image analysis. Conclusion PyRERT enables the results of the research group to be iteratively inherited in the form of software. It’s reusable basic classes and functional modules greatly improve the efficiency of scientific research task programming.