Abstract: This study proposed a vessel segmentation method based on Gabor features. According to the eigenvector of Hessian matrix of each pixel in the image, the vessel direction of each point was obtained to set the direction angle of Gabor filter, and the Gabor features of different vessel width at each point were extracted to establish the 6D vectors of each point. By reducing the dimension of the 6D vector, the 2D vector of each point was obtained and fused with the original image G channel. U-Net neural network was used to classify the fused image to segment vessels. The experimental results of this method in DRIVE dataset showed that this method had a good effect on the detection of small vessels and vessels at the intersection.