Abstract: A project of control of a high voltage pulse generator based on Arduino and its peripheral pulse sensor and temperature sensor was proposed for cell irreversible electroporation (IRE) test. By programming Arduino board, the analog photoelectric signal and the partial voltage signal of thermistor collected by pulse and temperature sensors were converted into digital signal and temperature value. The threshold of ECG R wave (>550) and temperature threshold (<37 oC) was set as trigger condition to control an 800 W high voltage pulse generator to release a fixed period pulse. Human lung cancer cells cultured in vitro were used to test and verify, and cell staining was used to evaluate the perforation. The results showed that Arduino and its sensors were sensitive to trigger and feedback. When the high voltage pulse generator was controlled to release 100 pulses with the parameters of 600 V, 1 200 V/cm and 100 ms pulse width, more than 95% of the cells showed nonthermal irreversible electroporation.