Abstract: Objective To establish the system software of multi-parameter monitoring by embedded Linux kernel and Qt library. Methods To determine the hardware system needed for the development of the system, carry out system Bootloader (Bootloader), Linux kernel, file system and Qt/Embedded (QtE) tailoring and transplantation and application development on the basis of the hardware system, and achieve the characteristic UI design. Results The changes of physiological parameters were observed in real time to improve the stability and real-time performance of the whole system and increase users' experience with QtE. Conclusion The embedded Linux+Qt multi-reference monitoring system can improve the stability, operability and functionality of real-time monitoring and multi-physiological information, and has good extensibility and maintainability.