Development of Colloidal Gold Detector Instrument Based on CMOS Image Processing
-
摘要: 该文分别从机械、硬件、软件等方面介绍了一种通过CMOS传感器采集图像，然后在Linux系统平台上运用图像处理算法分析免疫胶体金试纸显色反应的判读仪器。判读仪首先为每种测试项目单独设置并保存参数，然后测试项目样品时调用已保存的该项目参数进行测试，因此判读仪可以应用于多种领域多种项目。该文通过对临床中的CRP （C型反应性蛋白）标准样品进行定量实验测试，结果表明仪器拟合优度（R2=0.99），重复性大于93%。Abstract: This paper introduces a kind of immune colloidal gold detector instrument from the aspects of machinery,hardware and software.The instrument first collects one image through a CMOS sensor and then analyzes the image with image processing algorithm on Linux platform.Firstly,the instrument sets and stores the parameters separately for each test item,and then calls the saved item parameters when testing the item sample.So,the instrument can be used in a variety of fields and items.In this paper,a quantitative experimental test on C-reactive protein sample was performed,and the results indicate the coefficient of determination what denoted R2 equal to 0.99,and the repeatability is greater than 93%.
-
Keywords:
- colloidal gold /
- CMOS image sensor /
- quantitative test
-
-
[1] 陈俊彦, 肖琼. 免疫胶体金技术的应用与展望[J]. 化学分析计量, 2014, 31(1):101-104. [2] 刘星星, 郑永军, 李伶慧, 等. 多功能食品安全综合快速现场检测仪设计[J]. 食品安全质量检测学报, 2018, 9(4):868-877. [3] 毕建伟, 张明洲, 樊伟东. 基于CCD图像处理的胶体金试纸分析仪的设计[D]. 杭州:中国计量学院, 2016. [4] SPOELDER H J W. Virtual instrumentation and virtual environments[J]. IEEE Instrum Measur Mag,1999, 2(3):14-19.
[5] 刘蕾, 周蕾, 黄立华, 等. 基于一维CCD的免疫层析试纸条检测系统[J]. 仪器仪表学报, 2007, 28(2):246-251. [6] GONZALEZ R C, WOODS R E. 数字图像处理[M]. 阮秋琦, 等译. 北京:电子工业出版社, 2011.
计量
- 文章访问数: 8
- HTML全文浏览量: 0
- PDF下载量: 1
下载:
