Abstract: Objective To study and search for a balance between the image quality and acquisition speed in tomography of whole body bone scan. Methods Adjustments of acquisition conditions were carried out gradually every two months since April 2014. The qualities of fused SPECT/CT images were diagnosed by three doctors. Then the picture would be evaluated comprehensively by analyzing image quality and image resolution after adjusting image acquisition conditions. Results Seven kinds of image acquisition conditions taken were in line with diagnostic requirements. The third method is extended to clinical work best. Conclusion To obtain a high collection efficiency, parameters of bone tomography acquisition can be set a frame of 5 seconds, total 64 (5.625°), automatic probe close and continuous scanning. Also recommends the use of "continuous" instead of "step and shoot" approach in bone SPECT acquisition.