Abstract: To develop a method for determining complete degradation time of soluble hemostatic products in vitro with accuracy, high speed and effectiveness, the current weight loss method originated from GB/T 16886 serial standards was optimized by using dialysis bag combined with assay of reducing sugar. The degradation was carried out with 3% hydrogen peroxide solution, at 37℃, 150 rpm. The dialysis bags were taken out in the 3rd, 6th, 8th, 10th and 14th day, vacuum drying followed by percentage of weight loss testing. Cumulative content of reducing sugar in degradation solution out of dialysis bag was determined simultaneously. The correlation analysis was performed by SPSS 22.0 equipped with Pearson Correlation Coefficient. The correlation coefficient between percentage of weight loss and cumulative content of reducing sugar was 0.957, which illustrated high correlation with each other. Hence dialysis bag combined with assay of reducing sugar is capable of evaluating the degradation endpoint of soluble hemostatic products in vitro. The method also provides a way for the evaluation of degradation of other degradable biomaterials in vitro.