Abstract: One of the common critical complications in minimally invasive surgery is intraoperative and postoperative bleeding, particularly in laparoscopic procedures. Effective control of bleeding is essential for surgical success. Traditional hemostatic methods include packing and compression, high-frequency electrocoagulation, suturing, and interventional embolization. These approaches often cause significant tissue damage, involve complex procedures, and prove ineffective for diffuse bleeding or irregular wound surfaces. Portable in-situ electrospinning technology, an emerging nanofiber deposition technique independent of external high-voltage power sources, offers a revolutionary solution to these hemostatic challenges. This technology enables the real-time formation of nanofiber membranes over surgical wounds during procedures, precisely covering bleeding surfaces to achieve rapid, efficient, and biocompatible hemostasis and tissue repair. From a clinical application perspective, this paper systematically outlines the technology's characteristics, current development status, and its milestone significance for minimally invasive surgery. It further analyzes its application prospects, core advantages, and challenges, aiming to provide theoretical reference for its clinical translation.