Abstract: This study proposed and implemented a dual-mode focused ultrasound system for thermal ablation and histotripsy based on a single transducer. The acoustic characteristic measurements indicated that the maximum acoustic power was approximately 185W in thermal ablation mode and exceeded 6000W in histotripsy mode. Under different treatment parameters, the in vitro experimental results demonstrated that the system could achieve coagulative necrosis through thermal ablation and histotripsy to form homogenate and liquefaction zones, respectively. Therefore, this system can be used for preclinical research on the thermal and mechanical effects of focused ultrasound.