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      探究医用重组人源胶原蛋白功能敷料在伤口愈合中的作用

      Investigation on the Role of Medical Recombinant Human-Derived Collagen Functional Dressing in Wound Healing

        摘要
      • 摘要:
        目的 研究医用重组人源胶原蛋白功能敷料在伤口愈合中的生物学效应。
        方法 采用MTT法和RTCA法检测细胞毒性和细胞增殖，通过划痕实验和Transwell细胞迁移实验检测细胞的运动迁移能力，通过酶联免疫吸附测定法检测四种细胞上清中血管内皮生长因子（vascular endothelial growth factor, VEGF）、成纤维细胞生长因子（fibroblast growth factor, FGF）、CD31含量，动物手术后分别在1、4、13周后取手术创面进行苏木精-伊红（H&E）染色和免疫组化观察创面的炎症反应和CD31的表达情况。
        结果 医用重组人源胶原蛋白功能敷料促进细胞的增殖迁移，通过上调人真皮血管内皮细胞（human dermal vascular endothelial cells, HDVEC）、人血管内皮细胞（human vascular endothelial cells, HECV）中的VEGF、FGF和CD31的表达促进创面血管的新生，从而改善创面局部血供，调控创面的炎症反应，加速创面的愈合。
        结论 重组Ⅲ型人源化胶原蛋白在伤口愈合中发挥着重要的作用。

         

        Abstract:
        Objective To investigate the biological effect of medical recombinant human-derived collagen functional dressing in wound healing.
        Methods MTT assay and RTCA assay were used to detect cell toxicity and proliferation. Scratch assay and Transwell cell migration assay were used to detect cell motility and migration ability. Enzyme linked immunosorbent assay was used to detect the contents of VEGF, FGF, and CD31 in the supernatant of four types of cells. After animal surgery, the surgical wound was taken at 1 week, 4 weeks and 13 weeks, respectively, for hematoxylin eosin (H&E) staining and immunohistochemistry to observe the inflammatory response and CD31 expression of the wound.
        Results Medical recombinant human-derived collagen functional dressing promotes cell proliferation and migration, enhances wound angiogenesis by upregulating the expression of VEGF, FGF, and CD31 in HDVEC and HECV, thereby improving local blood supply to the wound, regulating the inflammatory response of the wound, and accelerating wound healing.
        Conclusion Recombinant type Ⅲ humanized collagen plays an important role in wound healing.

         

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