Abstract: This study aims to ensure the effectiveness of medical ultrasound diagnostic equipment in clinical applications by testing multiple key performance indicators using tissue-mimicking phantoms, and exploring the changes in these performance indicators over time. Firstly, 17 medical ultrasound diagnostic instruments in use were selected for research, and the depth of penetration, dead zone, lateral resolution, axial resolution, geometric position error, and cystic focal diameter error were tested according to the verification regulations. In addition, the study also retrospectively analyzed the performance testing data of 19 medical ultrasound diagnostic instruments over the past three consecutive years and conducted statistical analysis. Through the examination, all performance indicators of the 17 medical ultrasound diagnostic instruments meet the requirements of the verification regulations, indicating that they can provide high-quality ultrasound diagnostic images and data stably and reliably in clinical applications. Meanwhile, retrospective data shows that with the increase of service life, the resolution ability at the far field of 19 ultrasound diagnostic instruments has decreased, and the cystic focal diameter error has increased. Therefore, it is recommended that medical institutions establish a regular testing and maintenance system for medical ultrasound diagnostic equipment, and conduct regular performance evaluations and maintenance to ensure their good working condition.