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      基于微流控芯片的循环肿瘤细胞检测系统研究

      Study on Circulating Tumor Cell Detection System Based on Microfluidic Chip

        摘要
      • 摘要:
        目的 实现人体外周血液中循环肿瘤细胞（circulating tumor cells, CTCs）的高通量、高检出率的分选富集与检测，为癌症患者提供高效、精准的早期筛查。
        方法 设计一款分选富集与检测集成化的微流控芯片，采用荧光检测系统进行单细胞水平的CTCs检测，得到样本中的CTCs数量。
        结果 使用裂解红细胞后的外周血液样本进行6次实验，在进样速度达到0.2 mL/h时，CTCs能够达到78.6%的最佳检测率，组内相关系数达到0.8以上。
        结论 CTCs检测系统能够实现较高的检出率并具有良好的可靠性，可以为相关领域的临床研究提供可靠的参考。

         

        Abstract:
        Objective To achieve high throughput and high detection rate of circulating tumor cells (CTCs) in human peripheral blood, and to provide efficient and accurate early screening for cancer patients.
        Methods A microfluidic chip with the integration of sorting, enrichment and detection was designed, and CTCs at the single cell level were detected by fluorescence detection system to obtain the number of CTCs in samples.
        Results The peripheral blood samples after lysed red blood cells were used for 6 experiments. When the injection rate reached 0.2 mL/h, CTCs could reach the best detection rate of 78.6%, and the correlation coefficient within the group was above 0.8.
        Conclusion CTCs detection system can achieve high detection rate and has good reliability, which can provide a reliable reference for clinical research in related fields.

         

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